Abstract
The study was carried out, in the laboratory of Plant Virology, Plant Protection Department, College of Agriculture, University of Kerbala, to isolate and identify six isolates of the fungus Cladosporium sphaerospermum isolated from different environments (seeds, air, plant residues, and soil) in Al-Najaf province, Iraq. The fungal isolates were identified using the polymerase chain reaction (PCR) and determining the nucleotide sequence products of DNA using ITS1 and ITS4 primer pair. Results of analysis of the nucleotide sequences using BLAST (Basic Local Alignment Search Tool) showed that all the identified isolates of the fungus belong to C. sphaerospermum. A comparison of the nucleotide sequences with those available in the National Centre for Biotechnology Information (NCBI) revealed that all the identified C. sphaerospermum sequences were previously registered in NCBI.
