A thesis was discussed in the Department of Field Crops at the College of Agriculture at the University of Karbala (The effect of spraying with nano Moringa leaf extract on growth traits, yield, and expression of the CrtRB1 gene responsible for increasing β-Carotene in four genotypes of yellow maize Zea mayz L.)
. It also included the thesis titled (The effect of spraying with nano-moringa leaf extract on growth traits, yield, and expression of the CrtRB1 gene responsible for increasing β-Carotene in four genotypes of yellow corn. Zea mayz L.)
By student Amal Taher Odeh
Three chapters, namely the introduction and review of references, materials and methods of work, results and discussion, headed by Prof. Dr. Hamid Abd Khashan Al-Fartousi and membership of Prof. Dr. Abbas Ali Hussein Al-Amiri and Prof. Dr. Ziyad Hazem Jassab.
Under the supervision of Prof. Dr. Ali Nazim Farhoud, and in the presence of professors of the College of Agriculture, graduate students, and distinguished researchers. The message was intended to
- Study of the expression of the CrtRB1 gene, which is responsible for the synthesis of carotenoids, in four genotypes of yellow corn under spraying different concentrations of Moringa leaf nano extract.
- Determine the best concentration of nano-moringa leaf extract to obtain the highest yellow corn yield and the highest carotene content in the seeds.
- Study of the variation in genetic expression of the genes responsible for carotenoids between the (common) yellow genotype NadH315 and the dark red genotype NadH9055 using Next Generation Sequence technology. Moreover, the results were shown
1- It was noted that spraying with nano-moringa leaf extract can improve the growth characteristics, yield, and qualitative characteristics of yellow corn varieties.
3- The CrtRB1 gene has a role in increasing the content of the pigment β-carotene, by coupling the increased accumulation of β-carotene in grains with an increase in the expression of the CrtRB1 gene.
2- Through NGS, it becomes clear that there are other genes that contribute to the synthesis of carotenoids and β-Carotene, and these genes are distributed in different locations in the yellow maize genome.
