Molecular diagnosis and complete genome identification for the first time in Iraq of Fusarium solani FSSC5

A research team composed of master’s student Safa Jamil Sheehan and Prof. Raja Ghazi Abdel Mohsen and Prof. Dr. Adnan Abdul-Jalil Lahof in the Department of Plant Protection, College of Agriculture, University of Karbala, from the diagnosis and identification of the complete genome for the first time in Iraq of the fungus Fusarium solani FSSC5 that infects the ornamental plant known as cock’s mane (Celosia argentea) in many nurseries in the governorates of Karbala and Babylon, causing it a rot disease Coronary roots were also found to have a wide family range by causing the same disease to many economically important plant families. After studying the biological, pathological and phenotypic characteristics of the fungus, it was partially diagnosed using six genetic markers important in the diagnosis of fungi, including internal transcribed spacer (ITS), translation elongation factor (TEF-1α), RNA polymerase II (RPB 1) and RNA polymerase II (RPB 2). and beta-tubulin 1 (β-tubulin) as well as mitochondrial small-subunit ribosomal RNA. In order to determine the affiliation of F. solani isolated in this study to any of the 60 members of the Fusarium solani species complex (FSSC), which are taxonomic orders under the species, the entire genome of it was identified and interpreted by next generation sequencing technology. It was found that it consists of 51,743,296 nitrogen bases, and the number of genes encoding proteins is 17,940 genes encoding 17,654 proteins, and it belongs to the fifth member (FSSC5). JAMJFY000000.1