Entitled:
“Evaluation of the Effect of the Growth Regulator DTA-6 on DOF4 Gene Expression and the Agronomic and Biochemical Traits of Two Soybean Cultivars”
A master’s thesis entitled “Evaluation of the Effect of the Growth Regulator DTA-6 on DOF4 Gene Expression and the Agronomic and Biochemical Traits of Two Soybean Cultivars”
by the student Huda Hussein Naama was discussed at the College of Agriculture, University of Karbala.
The thesis is composed of seven chapters: Abstract, Introduction, Literature Review, Materials and Methods, Results, Conclusions and Recommendations, and References.
The study aimed to:
- Evaluate the effect of foliar application of the growth regulator DTA-6 on the growth characteristics and yield of two soybean cultivars.
- Analyze the influence of DTA-6 on the chemical and biochemical properties of the seeds, with a focus on protein content, oil percentage, and fatty acid composition.
- Investigate the effect of DTA-6 on the relative expression levels of the DOF4 gene to determine its role in enhancing physiological traits and seed quality.
- Compare the response of the two cultivars to identify genetic and physiological differences affecting yield performance and quality.
- Determine the optimal timing for applying DTA-6—between the branching stage (GS-22) and the flowering stage (GS-51)—to achieve the best outcomes in terms of growth, yield, and quality.
Based on the findings, the student concluded the following:
• The growth regulator DTA-6 significantly induced the relative expression of the DOF4 gene.
• DTA-6 demonstrated high efficacy in improving vegetative growth traits in both cultivars, Shaymaa and Ibaa.
• The cultivar Shaymaa outperformed in most quality-related traits.
• DTA-6 had a significant positive effect on improving yield components.





